Abstract: Aiming at the problems of low imaging contrast and difficult recognition of traditional microscopes in low-illuminance environments, a continuous zoom polarization video microscope objective in combination was designed with division-of-focal-plane polarization imaging technique. The objective lens worked in the visible light band, with a zoom range of 1×−6.5×, an object-line field of view of 1.70−11.1 mm, a total length of 220 mm, a working distance of 22 mm, and image distance of 14 mm. And the system was followed by a polarization detector to obtain the target’s polarization information. After simulation analysis, the image plane was stable in the zoom range, and the imaging quality was close to the diffraction limit in each zoom state. The system can not only obtain images of different magnifications of the target on the electronic display and alleviate visual fatigue, but also obtain the polarization information of the target, improve the recognition probability of the target, the observation efficiency and the imaging quality in the case of low illumination.