Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar

Yang Hui; Zhao Xuesong; Sun Yanfei; Wang Tiedong; Ye Jiesong

doi:10.3788/IRLA201767.1030004

Volume 46 Issue 10

Nov. 2017

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Yang Hui, Zhao Xuesong, Sun Yanfei, Wang Tiedong, Ye Jiesong. Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar[J]. Infrared and Laser Engineering, 2017, 46(10): 1030004-1030004(8). doi: 10.3788/IRLA201767.1030004

Citation:

Yang Hui, Zhao Xuesong, Sun Yanfei, Wang Tiedong, Ye Jiesong. Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar[J]. Infrared and Laser Engineering, 2017, 46(10): 1030004-1030004(8). doi: 10.3788/IRLA201767.1030004

Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar

doi: 10.3788/IRLA201767.1030004

1.
Army Officer Academy,The Chinese People's Liberation Army,Hefei 230031,China;
2.
Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Hefei 230031,China

Received Date: 2017-02-10
Rev Recd Date: 2017-03-20
Publish Date: 2017-10-25

Abstract

Based on two independent physical phenomena:laser induced fluorescence(LIF) and depolarization resulting from elastic scattering on non-spherical particles, a short range lidar system for real-time standoff detection of bio-agents was developed. The lidar system included three laser sources, two receiving telescopes, one depolarization component and fluorescence spectral signature analyzing spectrograph. It was designed to provide the stand-off detection capability at ranges from 200 m up to several kilometers. For fluorescence excitation, 3rd (355 nm) and 4th (266 nm) harmonics of Nd:YAG pulsed lasers were used. They emitted short (about 6 ns) pulses with the repetition frequency of 20 Hz. Collecting optical system for fluorescence echo detection and spectral content analysis included 25.0 mm diameter f/4 Newton telescope, Czerny Turner spectrograph and a 32-channel PMT. The depolarization and Mie echo signal were collected by a Cassegrain telescope with an aperture diameter of 12.5 mm. Through the simulative calculation of SNR of fluorescence measurement, it was found that, with the minimum detectable SNR value of 10 as reference, the bio-agent cloud with concentration of 10 000ACPLA at the distance of 1 km could not be detected in daytime, while a rather good signal intensity could be obtained in nighttime. The preliminary analysis to the depolarization measurement results indicated that:(1) the depolarization ratios were wavelength-dependent; (2) depolarization measurement using multiple wavelengths could increase discrimination efficiency significantly.
- bio-agents,
- lidar,
- fluorescence,
- depolarization ratio,
- measurement,
- SNR

References

[1]	Zhao Yiming, Jiang Yuesong, Lu Xiaomei. Theory analysis of polarization character istic of the light scattered by the aerosol[J]. Infrared and Laser Engineering, 2007, 36(6):862-865. (in Chinese)赵一鸣, 江月松, 路小梅. 气溶胶散射光偏振度特性的理论研究[J]. 红外与激光工程, 2007, 36(6):862-865.
[2]	Zou Bingfang, Zhang Yinchao. Multi-wavelength fluorescence lidar detection of bioaerosols[J]. Infrared and Laser Engineering, 2006, 35(S3):262-267. (in Chinese)邹炳芳, 张寅超. 多波长荧光激光雷达测生物气溶胶的数值模拟计算[J]. 红外与激光工程, 2006, 35(S3):262-267.
[3]	Lan Tiange, Xiong Wei, Fang Yonghua, et al. Study on passive detection of biological aerosol with Fourier-transform infrared spectctroscopic technique[J]. Acta Optica Sinica, 2010, 30(6):1656-1661. (in Chinese)兰天鸽, 熊伟, 方勇华, 等. 应用被动傅里叶变换红外光谱技术探测生物气溶胶研究[J]. 光学学报, 2010, 30(6):1656-1661.
[4]	Cai Shuyao, Zhang Pei, Zhu Linglin, et al. Research on detection technology of bioaerosols with tryptophan intrinstic fluorescence measurement[J]. Acta Optica Sinica, 2012, 32(5):0512009-1-6. (in Chinese)蔡淑窈, 张佩, 朱玲琳, 等. 基于色氨酸本征荧光测量的生物气溶胶检测技术研究[J]. 光学学报, 2012, 32(5):0512009-1-6.
[5]	Gu Youlin, Wang Cheng, Yang Li, et al. Infrared extinction before and after aspergillus niger spores inactivation[J]. Infrared and Laser Engineering, 2015, 44(1):36-41. (in Chinese)顾有林, 王成, 杨丽, 等. 黑曲霉孢子灭活前后红外消光特性[J]. 红外与激光工程, 2015, 44(1):36-41.
[6]	Wu Taihu, Mao Jiawen, Chen Feng, et al. Rapid trace microbia detection system[J]. Optical and Precision Engereering, 2015, 23(11):3061-3068. (in Chinese)吴太虎, 毛佳文, 陈锋, 等. 痕量微生物快速检测系统[J]. 光学精密工程, 2015, 23(11):3061-3068.
[7]	Lakowicz J R. Principles of Fluorescence Spectroscopy[M]. New York:Kluwer Academic/Plenum Publisher, 1999.
[8]	Yang Hui, Xiao Xue, Zhao Xuesong, et al. Study on fluorescence spectra of thiamine, riboflavin and pyridoxine[C]//SPIE, 2015, 9903:99030H-1-17.
[9]	Measures R M. Laser Remote Sensing. Fundamentals and applications[M]. New York:Krieger Publishing Company, 1992.
[10]	Simard J R, Roy G, Mathieu P, et al. Standoff sensing of bioaerosols using intensified range-gated spectral analysis of laser-induced fluorescence[J]. IEEE Trans on Geoscience and Remote Sensing, 2004, 42:865-873.
[11]	Agishev R, Gross B, Moshary F, et al. Simple approach to predict APD/PMT lidar detector performance under sky background using dimensionless parametrization[J]. Optics and Lasers in Engineering, 2006, 44:779-796.
[12]	Schotland R M, Sassen K, Stone R. Observation by lidar of linear depolarization ratios for hydrometeors[J]. J Appl Meteorol, 1971, 10:1011-1017.
[13]	Gimmestad G G. Reexamination of depolarization in lidar measurements[J]. Appl Opt, 2008, 47:3795-3802.
[14]	Liu Dong, Tao Zongming, Wu Decheng, et al. Development of three-wavelength-Raman-polarization lidar system and case study[J]. Acta Optica Sinica, 2013, 33(2):0228001-1-6. (in Chinese)刘东, 陶宗明, 吴德成, 等. 三波长拉曼偏振激光雷达系统研制及探测个例[J]. 光学学报, 2013, 33(2):0228001-1-6.
[15]	Yang Zijian. Construction and key technology research of biological aerosol monitoring system based on laser radar technology[D]. Beijing:Military Medical Science Academy of the PLA, 2015. (in Chinese)杨子健. 基于激光雷达技术的生物气溶胶监测系统构建与关键技术研究[D]. 北京:中国人民解放军军事医学科学院, 2015.
[16]	Han Xue. Depolarization characteristic of aerosolscattering in laser research[D]. Changchun:Changchun University of Science and Technology, 2012. (in Chinese)韩雪. 气溶胶散射对激光退偏特性的研究[D]. 长春:长春理工大学, 2012.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar

1. Army Officer Academy,The Chinese People's Liberation Army,Hefei 230031,China;

2. Anhui Institute of Optics and Fine Mechanics,Chinese Academy of Sciences,Hefei 230031,China

Received Date: 2017-02-10

Rev Recd Date: 2017-03-20

Publish Date: 2017-10-25

Key words:

Abstract: Based on two independent physical phenomena:laser induced fluorescence(LIF) and depolarization resulting from elastic scattering on non-spherical particles, a short range lidar system for real-time standoff detection of bio-agents was developed. The lidar system included three laser sources, two receiving telescopes, one depolarization component and fluorescence spectral signature analyzing spectrograph. It was designed to provide the stand-off detection capability at ranges from 200 m up to several kilometers. For fluorescence excitation, 3rd (355 nm) and 4th (266 nm) harmonics of Nd:YAG pulsed lasers were used. They emitted short (about 6 ns) pulses with the repetition frequency of 20 Hz. Collecting optical system for fluorescence echo detection and spectral content analysis included 25.0 mm diameter f/4 Newton telescope, Czerny Turner spectrograph and a 32-channel PMT. The depolarization and Mie echo signal were collected by a Cassegrain telescope with an aperture diameter of 12.5 mm. Through the simulative calculation of SNR of fluorescence measurement, it was found that, with the minimum detectable SNR value of 10 as reference, the bio-agent cloud with concentration of 10 000ACPLA at the distance of 1 km could not be detected in daytime, while a rather good signal intensity could be obtained in nighttime. The preliminary analysis to the depolarization measurement results indicated that:(1) the depolarization ratios were wavelength-dependent; (2) depolarization measurement using multiple wavelengths could increase discrimination efficiency significantly.

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Reference (16)

[1]	Zhao Yiming, Jiang Yuesong, Lu Xiaomei. Theory analysis of polarization character istic of the light scattered by the aerosol[J]. Infrared and Laser Engineering, 2007, 36(6):862-865. (in Chinese)赵一鸣, 江月松, 路小梅. 气溶胶散射光偏振度特性的理论研究[J]. 红外与激光工程, 2007, 36(6):862-865.
[2]	Zou Bingfang, Zhang Yinchao. Multi-wavelength fluorescence lidar detection of bioaerosols[J]. Infrared and Laser Engineering, 2006, 35(S3):262-267. (in Chinese)邹炳芳, 张寅超. 多波长荧光激光雷达测生物气溶胶的数值模拟计算[J]. 红外与激光工程, 2006, 35(S3):262-267.
[3]	Lan Tiange, Xiong Wei, Fang Yonghua, et al. Study on passive detection of biological aerosol with Fourier-transform infrared spectctroscopic technique[J]. Acta Optica Sinica, 2010, 30(6):1656-1661. (in Chinese)兰天鸽, 熊伟, 方勇华, 等. 应用被动傅里叶变换红外光谱技术探测生物气溶胶研究[J]. 光学学报, 2010, 30(6):1656-1661.
[4]	Cai Shuyao, Zhang Pei, Zhu Linglin, et al. Research on detection technology of bioaerosols with tryptophan intrinstic fluorescence measurement[J]. Acta Optica Sinica, 2012, 32(5):0512009-1-6. (in Chinese)蔡淑窈, 张佩, 朱玲琳, 等. 基于色氨酸本征荧光测量的生物气溶胶检测技术研究[J]. 光学学报, 2012, 32(5):0512009-1-6.
[5]	Gu Youlin, Wang Cheng, Yang Li, et al. Infrared extinction before and after aspergillus niger spores inactivation[J]. Infrared and Laser Engineering, 2015, 44(1):36-41. (in Chinese)顾有林, 王成, 杨丽, 等. 黑曲霉孢子灭活前后红外消光特性[J]. 红外与激光工程, 2015, 44(1):36-41.
[6]	Wu Taihu, Mao Jiawen, Chen Feng, et al. Rapid trace microbia detection system[J]. Optical and Precision Engereering, 2015, 23(11):3061-3068. (in Chinese)吴太虎, 毛佳文, 陈锋, 等. 痕量微生物快速检测系统[J]. 光学精密工程, 2015, 23(11):3061-3068.
[7]	Lakowicz J R. Principles of Fluorescence Spectroscopy[M]. New York:Kluwer Academic/Plenum Publisher, 1999.
[8]	Yang Hui, Xiao Xue, Zhao Xuesong, et al. Study on fluorescence spectra of thiamine, riboflavin and pyridoxine[C]//SPIE, 2015, 9903:99030H-1-17.
[9]	Measures R M. Laser Remote Sensing. Fundamentals and applications[M]. New York:Krieger Publishing Company, 1992.
[10]	Simard J R, Roy G, Mathieu P, et al. Standoff sensing of bioaerosols using intensified range-gated spectral analysis of laser-induced fluorescence[J]. IEEE Trans on Geoscience and Remote Sensing, 2004, 42:865-873.
[11]	Agishev R, Gross B, Moshary F, et al. Simple approach to predict APD/PMT lidar detector performance under sky background using dimensionless parametrization[J]. Optics and Lasers in Engineering, 2006, 44:779-796.
[12]	Schotland R M, Sassen K, Stone R. Observation by lidar of linear depolarization ratios for hydrometeors[J]. J Appl Meteorol, 1971, 10:1011-1017.
[13]	Gimmestad G G. Reexamination of depolarization in lidar measurements[J]. Appl Opt, 2008, 47:3795-3802.
[14]	Liu Dong, Tao Zongming, Wu Decheng, et al. Development of three-wavelength-Raman-polarization lidar system and case study[J]. Acta Optica Sinica, 2013, 33(2):0228001-1-6. (in Chinese)刘东, 陶宗明, 吴德成, 等. 三波长拉曼偏振激光雷达系统研制及探测个例[J]. 光学学报, 2013, 33(2):0228001-1-6.
[15]	Yang Zijian. Construction and key technology research of biological aerosol monitoring system based on laser radar technology[D]. Beijing:Military Medical Science Academy of the PLA, 2015. (in Chinese)杨子健. 基于激光雷达技术的生物气溶胶监测系统构建与关键技术研究[D]. 北京:中国人民解放军军事医学科学院, 2015.
[16]	Han Xue. Depolarization characteristic of aerosolscattering in laser research[D]. Changchun:Changchun University of Science and Technology, 2012. (in Chinese)韩雪. 气溶胶散射对激光退偏特性的研究[D]. 长春:长春理工大学, 2012.

Bio-agents and aerosol measurement by fluorescence and depolarization short-distance lidar

doi: 10.3788/IRLA201767.1030004

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References

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通讯作者: 陈斌, bchen63@163.com

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