Infrared extinction before and after aspergillus niger spores inactivation

Gu Youlin; Wang Cheng; Yang Li; Ou Zongwei; Hu Yihua; Li Le; Zhao Yizheng; Chen Wei; Wang Peng

Volume 44 Issue 1

Feb. 2015

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Gu Youlin, Wang Cheng, Yang Li, Ou Zongwei, Hu Yihua, Li Le, Zhao Yizheng, Chen Wei, Wang Peng. Infrared extinction before and after aspergillus niger spores inactivation[J]. Infrared and Laser Engineering, 2015, 44(1): 36-41.

Citation:

Gu Youlin, Wang Cheng, Yang Li, Ou Zongwei, Hu Yihua, Li Le, Zhao Yizheng, Chen Wei, Wang Peng. Infrared extinction before and after aspergillus niger spores inactivation[J]. Infrared and Laser Engineering, 2015, 44(1): 36-41.

Infrared extinction before and after aspergillus niger spores inactivation

Gu Youlin^1,2,
Wang Cheng²,
Yang Li²,
Ou Zongwei²,
Hu Yihua^1,2,
Li Le^1,2,
Zhao Yizheng^1,2,
Chen Wei^1,2,
Wang Peng³

1.
State Key Laboratory of Pulsed Power Laser TechnologyElectronic Engineering Institute,Hefei 230037,China;
2.
Electronic Engineering Institute,Hefei 230037,China;
3.
Key Laboratory of Ion Beam EngineeringChinese Academy of Sciences,Hefei 230031,China

Received Date: 2014-05-11
Rev Recd Date: 2014-06-17
Publish Date: 2015-01-25

Abstract

Aspergillus niger spores is an important part of bio-aerosols, and its mass extinction coefficient is an important parameter for study on electromagnetic attenuation characteristics of aspergillus niger spores. The reflection spectra before and after aspergillus niger spores inactivation within the 2.5-15 um waveband were measured by squash method. The complex refractive index of aspergillus niger spores in the infrared waveband was calculated by using Kramers-Kroning (K-K) relation. Then, based on Mie scattering theory, the mass extinction coefficient before and after aspergillus niger spores inactivation in the infrared waveband were obtained, and the results were analyzed and discussed. The results show that average mass extinction coefficient has a decrease of 4.6 percent in the 3-5 um waveband and a decrease of 89.5 percent in the 8-14 um waveband. In light of this, to retain activity of aspergillus niger spores will play an important role in the increasing of its electromagnetic attenuation abilities.
- aspergillus niger spores,
- infrared,
- inactivation,
- complex refractive index,
- mass extinction coefficient

References

[1]	Feng Mingchun, Xu Liang, Gao Minguang, et al. Optical properties research of Bacillus subtilis spores by fourier transform infrared spectroscopy[J]. Spectroscopy and Spectral Analysis, 2012, 32(12): 3193-3196. (in Chinese) 冯明春, 徐亮, 高闽光, 等. 傅里叶红外变换光谱仪对枯草芽孢杆菌的光学特性研究[J]. 光谱学与光谱析, 2012, 32 (12): 3193-3196.
[2]
[3]
[4]	Dalterio R A, Nelson W H, Britt D, et al. Steady-state and decay characteristics of protein tryptophan fluorescence from bacteria[J]. Applied spectroscopy, 1986, 40(1): 86-90.
[5]	Bronk Burt V, Reinisch Lou. Variability of steady-state bacterial fluorescence with respect to growth conditions [J]. Applied Spectroscopy, 1993, 47(4): 436-440.
[6]
[7]
[8]	Pinnick Ronald G, Hill Steven C, Nachman Paul, et al. Fluorescence particle counter for detecting airborne bacteria and other biological particles [J]. Aerosol Science and Technology, 1995, 23(4): 653-664.
[9]
[10]	Paul Nachman, Gang Chen, Pinnick R G, et al. Conditional-sampling spectrograph detection system for fluorescence measurements of individual airborne biological particles [J]. Applied Optics, 1996, 35(7): 1069-1076.
[11]
[12]	Ross K F A, Billing E V E. The water and solid content of living bacterial spores and vegetative cells as indicated by refractive index measurement[J]. J Gen Microbiol, 1957, 16 (2):418-425.
[13]
[14]	Zou Bingfang, Zhang Yinchao. Multi-wavelength fluorescence lidar detection of bioaerosols [J]. Infrared and Laser Engineering, 2006, 35(S3): 262-267. (in Chinese) 邹炳芳, 张寅超. 多波长荧光激光雷达测生物气溶胶的数值模拟计算[J]. 红外与激光工程, 2006, 35(S3): 262-267.
[15]
[16]	Lan Tiange, Xiong Wei, Fang Yonghua, et al. Study on passive detection of biological aerosol with Fourier-transform infrared spectctroscopic technique [J]. Acta Optica Sinica, 2010, 30(6): 1656-1661. (in Chinese) 兰天鸽, 熊伟, 方勇华, 等. 应用被动傅里叶变换红外光谱技术探测生物气溶胶研究[J]. 光学学报, 2010, 30(6): 1656.-1661
[17]	Feng Chunxia, Huang Lihua, Zhou Guangchao, et al. Computation and analysis of light scattering by monodisperse biological aerosols [J]. Chinese Journal of Lasers, 2010, 37 (10): 2592-2598. (in Chinese) 冯春霞, 黄立华, 周光超, 等. 单分散生物气溶胶光散射特性的计算与分析[J]. 中国激光, 2010, 37(10): 2592-2598.
[18]
[19]
[20]	Cai Shuyao, Zhang Pei, Zhu Linglin, et al. Research on detection technology of bioaerosols with tryptophan intrinstic fluorescence measurement [J]. Acta Optica Sinica, 2012, 32 (5): 0512009-1-0512009-6. (in Chinese) 蔡淑窈, 张佩, 朱玲琳, 等. 基于色氨酸本征荧光测量的生物气溶胶检测技术研究[J]. 光学学报, 2012, 32(5): 0512009-1-0512009-6.
[21]	Tuminello P S, Arakawa E T, Khare B N, et al. Optical properties of Bacillus subtilis spores from 0.2 to 2.5 um[J]. Appl Opt, 1997, 36(13): 2818-2824.
[22]
[23]	Sun Dujuan, Hu Yihua, Gu Youlin, et al. Preparation and performance testing of metallic biologic particles [J]. Acta Photonica Sinica, 2013, 42(5): 555-558. (in Chinese) 孙杜娟, 胡以华, 顾有林, 等. 金属化生物颗粒的制备与性能测试[J]. 光子学报, 2013, 42(5): 555-558.
[24]
[25]	Sun Dujuan, Hu Yihua, Gu Youlin, et al. Determination and model construction of microbes'complex refractive index in far infrared band[J]. Acta Phys Sin, 2013, 62(9): 094218-1-094218-9. (in Chinese) 孙杜娟, 胡以华, 顾有林, 等. 微生物远红外波段复折射率测定及模型构建[J]. 物理学报, 2013, 62(9): 094218-1-094218-9.
[26]
[27]	Michael I M, Larry D T, Andew A L. Scattering, Absorption and Emission of Light by Small Particles [M]. London: Cambridge, 2002: 3-8.
[28]
[29]	Lucarini V, Saarinen J J, PeiponenK E, et al. Kramers-Kronig Relations in Optical Meterials Research [M]. Germany:Springer-Verlag Berlin Heidelberg, 2005: 9-25.
[30]
[31]	Dou Zhengwei, Li Xiaoxia, Zhao Jijin. Complex refraction indices of expanded graphite deduced from its refrection spectra in infraredband [J]. Acta Armamentar II, 2011, 32 (4): 498-502. (in Chinese) 豆正伟, 李晓霞, 赵纪金. 光谱法研究膨胀石墨红外波段复折射率[J]. 兵工学报, 2011, 32(4): 498-502.
[32]
[33]	Bohern C F, Huffman D R. Absorption and Scattering of Light by Small Particles [M]. New York: John Wiley Sons, 1983: 227-234.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Infrared extinction before and after aspergillus niger spores inactivation

1. State Key Laboratory of Pulsed Power Laser TechnologyElectronic Engineering Institute,Hefei 230037,China;

2. Electronic Engineering Institute,Hefei 230037,China;

3. Key Laboratory of Ion Beam EngineeringChinese Academy of Sciences,Hefei 230031,China

Received Date: 2014-05-11

Rev Recd Date: 2014-06-17

Publish Date: 2015-01-25

Key words:

Abstract: Aspergillus niger spores is an important part of bio-aerosols, and its mass extinction coefficient is an important parameter for study on electromagnetic attenuation characteristics of aspergillus niger spores. The reflection spectra before and after aspergillus niger spores inactivation within the 2.5-15 um waveband were measured by squash method. The complex refractive index of aspergillus niger spores in the infrared waveband was calculated by using Kramers-Kroning (K-K) relation. Then, based on Mie scattering theory, the mass extinction coefficient before and after aspergillus niger spores inactivation in the infrared waveband were obtained, and the results were analyzed and discussed. The results show that average mass extinction coefficient has a decrease of 4.6 percent in the 3-5 um waveband and a decrease of 89.5 percent in the 8-14 um waveband. In light of this, to retain activity of aspergillus niger spores will play an important role in the increasing of its electromagnetic attenuation abilities.

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Reference (33)

[1]	Feng Mingchun, Xu Liang, Gao Minguang, et al. Optical properties research of Bacillus subtilis spores by fourier transform infrared spectroscopy[J]. Spectroscopy and Spectral Analysis, 2012, 32(12): 3193-3196. (in Chinese) 冯明春, 徐亮, 高闽光, 等. 傅里叶红外变换光谱仪对枯草芽孢杆菌的光学特性研究[J]. 光谱学与光谱析, 2012, 32 (12): 3193-3196.
[2]
[3]
[4]	Dalterio R A, Nelson W H, Britt D, et al. Steady-state and decay characteristics of protein tryptophan fluorescence from bacteria[J]. Applied spectroscopy, 1986, 40(1): 86-90.
[5]	Bronk Burt V, Reinisch Lou. Variability of steady-state bacterial fluorescence with respect to growth conditions [J]. Applied Spectroscopy, 1993, 47(4): 436-440.
[6]
[7]
[8]	Pinnick Ronald G, Hill Steven C, Nachman Paul, et al. Fluorescence particle counter for detecting airborne bacteria and other biological particles [J]. Aerosol Science and Technology, 1995, 23(4): 653-664.
[9]
[10]	Paul Nachman, Gang Chen, Pinnick R G, et al. Conditional-sampling spectrograph detection system for fluorescence measurements of individual airborne biological particles [J]. Applied Optics, 1996, 35(7): 1069-1076.
[11]
[12]	Ross K F A, Billing E V E. The water and solid content of living bacterial spores and vegetative cells as indicated by refractive index measurement[J]. J Gen Microbiol, 1957, 16 (2):418-425.
[13]
[14]	Zou Bingfang, Zhang Yinchao. Multi-wavelength fluorescence lidar detection of bioaerosols [J]. Infrared and Laser Engineering, 2006, 35(S3): 262-267. (in Chinese) 邹炳芳, 张寅超. 多波长荧光激光雷达测生物气溶胶的数值模拟计算[J]. 红外与激光工程, 2006, 35(S3): 262-267.
[15]
[16]	Lan Tiange, Xiong Wei, Fang Yonghua, et al. Study on passive detection of biological aerosol with Fourier-transform infrared spectctroscopic technique [J]. Acta Optica Sinica, 2010, 30(6): 1656-1661. (in Chinese) 兰天鸽, 熊伟, 方勇华, 等. 应用被动傅里叶变换红外光谱技术探测生物气溶胶研究[J]. 光学学报, 2010, 30(6): 1656.-1661
[17]	Feng Chunxia, Huang Lihua, Zhou Guangchao, et al. Computation and analysis of light scattering by monodisperse biological aerosols [J]. Chinese Journal of Lasers, 2010, 37 (10): 2592-2598. (in Chinese) 冯春霞, 黄立华, 周光超, 等. 单分散生物气溶胶光散射特性的计算与分析[J]. 中国激光, 2010, 37(10): 2592-2598.
[18]
[19]
[20]	Cai Shuyao, Zhang Pei, Zhu Linglin, et al. Research on detection technology of bioaerosols with tryptophan intrinstic fluorescence measurement [J]. Acta Optica Sinica, 2012, 32 (5): 0512009-1-0512009-6. (in Chinese) 蔡淑窈, 张佩, 朱玲琳, 等. 基于色氨酸本征荧光测量的生物气溶胶检测技术研究[J]. 光学学报, 2012, 32(5): 0512009-1-0512009-6.
[21]	Tuminello P S, Arakawa E T, Khare B N, et al. Optical properties of Bacillus subtilis spores from 0.2 to 2.5 um[J]. Appl Opt, 1997, 36(13): 2818-2824.
[22]
[23]	Sun Dujuan, Hu Yihua, Gu Youlin, et al. Preparation and performance testing of metallic biologic particles [J]. Acta Photonica Sinica, 2013, 42(5): 555-558. (in Chinese) 孙杜娟, 胡以华, 顾有林, 等. 金属化生物颗粒的制备与性能测试[J]. 光子学报, 2013, 42(5): 555-558.
[24]
[25]	Sun Dujuan, Hu Yihua, Gu Youlin, et al. Determination and model construction of microbes'complex refractive index in far infrared band[J]. Acta Phys Sin, 2013, 62(9): 094218-1-094218-9. (in Chinese) 孙杜娟, 胡以华, 顾有林, 等. 微生物远红外波段复折射率测定及模型构建[J]. 物理学报, 2013, 62(9): 094218-1-094218-9.
[26]
[27]	Michael I M, Larry D T, Andew A L. Scattering, Absorption and Emission of Light by Small Particles [M]. London: Cambridge, 2002: 3-8.
[28]
[29]	Lucarini V, Saarinen J J, PeiponenK E, et al. Kramers-Kronig Relations in Optical Meterials Research [M]. Germany:Springer-Verlag Berlin Heidelberg, 2005: 9-25.
[30]
[31]	Dou Zhengwei, Li Xiaoxia, Zhao Jijin. Complex refraction indices of expanded graphite deduced from its refrection spectra in infraredband [J]. Acta Armamentar II, 2011, 32 (4): 498-502. (in Chinese) 豆正伟, 李晓霞, 赵纪金. 光谱法研究膨胀石墨红外波段复折射率[J]. 兵工学报, 2011, 32(4): 498-502.
[32]
[33]	Bohern C F, Huffman D R. Absorption and Scattering of Light by Small Particles [M]. New York: John Wiley Sons, 1983: 227-234.

Infrared extinction before and after aspergillus niger spores inactivation

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