Abstract: Color cameras are widely used in the printing industry, graphic arts, medical area, environment and many other applications. In order to save cost and maintain high colorimetric accuracy, a method that utilizing a single-channel pre-filter to calibrate color camera's spectral sensitivity curve was put forward. The pre-filter's simulation accuracy depended on the degree of matching Luther conditions, and it was impossible to match perfectly due to the limitation filter material, the film design, and the nonideal filter fabrication. The goal of this work was to design the target curve according to analyzing the influence factor of the simulate accuracy, using the continuously tunable monochromatic light source system to test the color CCD relative spectral response, selecting appropriate evaluation function model to simulate theoretical spectral transmittance, referring the CIE standard test colors and using CIE2000 color formula to evaluate the theoretical color error. This work shows that after increasing the pre-filter, the mean change of the color error is about 73%, and the color effect of the camera has been enhanced significantly.