Abstract: The high performance phosphor screen can effectively enhance the performance of low light level image intensifiers. The performance of phosphor screen mainly depends on the nature of the powder layer. The powder layer is essentially formed by the accumulation of phosphor grains, and therefore the related character research of the powder layer based on powder stacking theory is helpful to guide the actual production process to obtain higher quality powder layer, thereby enhancing the performance of the screen. Therefore, a theoretical analysis of the powder layer density based on the classic powder stacking theory was made, and combined with the actual production, some further related studies for the best powder layer thickness were obtained, taking a certain type of phosphor for example. The result shows that the powder layer first should be possible to achieve the optimum thickness, and then make the density to the maximum, so can obtain the better powder layer, hence, the performance of phosphor screen will get better.