Abstract: Analytical accuracy of polarization remote sensing system is determined by instrumental detection performance. However, it is limited for the instrumental polarization introduced by various factors. In this paper, a multi-point calibration method for measurement matrix of division-of-amplitude (DoA) photopolarimetric detection system had been proposed by analyzing the measurement principle, and the conditions to calibrate a DoA polarization detection system with interval sampling polarization azimuth angle had been derived. Through building a test platform and conducting comparative experiments, the measuring matrix of aviation multi-angular polarimetric radiometer (AMPR) had been determined using the multi-point calibration method mentioned above and the uncertainty had been investigated. The experiment results indicate the measurement error of AMPR is less than 0.5% and corresponding uncertainty is also less than 0.5%, which demonstrate the detection system requirements has been met for AMPR and the feasibility of multi-point calibration method is verified.