Abstract: Pointing accuracy is the most important parameter to evaluate the performance of the star camera. Laboratory accuracy determination methods for sub-arcsec star camera were presented. Temporal error (TE) was tested with static multiple stars target and collimator considering real signal level of the star camera. Stewart platform was used to adjust the camera with micro-steps, inter-frame difference method was applied to obtain relative distance of tie-points, then high spatial frequency error (HSFE) was calculated with statistics method with the benefit of avoiding planning special paths. Low spatial frequency error (LSFE) introduced by chromatic aberration was measured equivalently with different kind of optical filters; moreover, LSFE in distortion calibration and target measurement was calculated. Test is taken and the result is in line with design value, the validity of the above methods is verified.