Abstract: Irradiation experiments with a 1 064 nm pulse laser were conducted to investigate the image interrupt effect of pulse laser irradiating CMOS camera with rolling shutter. Image interrupt effects were observed in the experimental results with bright area and dark area in the vertical direction. Calculation results show that the number of dark area rows is a constant. According to the integrating mode and scanning mode of CMOS image sensor, the formation mechanism of image interrupt effect is the storage asynchronism of CMOS with rolling shutter. Theoretical calculation value fit well with the experimental result. The reason of the existing of image without dark area was also theoretically explained. Method based on stitching several interrupted images was proposed to obtain a full image without interruption, which was very similar to the experimental imaging results.