Abstract: Optical positioning technology has been widely used in surgical navigation, target tracking and virtual reality systems, in order to improve the accuracy of positioning, many research work has been done on signal measurement and localization algorithms. Using this system, the quality of the light source used for optical positioning was improved by screening near-infrared LED, and the good conditions were created for accurate positioning. The screening detection system consisted of an optical performance detecting section and a high and low temperature testing section. By controlling the rotation angles of the pitch axis and the rolling axis in the turntable, the radiation intensity of the light-emitting elements could be continuously measured by photodetector, the radiation data of near-infrared LED at different rotating faces and different longitudinal angles was obtained, and the half power angle value could be calculated. According to the two important indicators of luminous intensity and half power angle, first, the extreme value data is removed from the selected group; and then two near-infrared LEDs with relative deviation within 5% were selected; and finally the high-low temperature impact test was performed. The selected near-infrared LEDs have stable performance and good luminous consistency, and can be used in groups.