Abstract: The three-dimensional measurement of human body posture is of great significance to the comfort evaluation of car seat design. In order to acquire the 3D data of the human body in the car quickly and accurately, a method of 3D data acquisition based on binocular vision was adopted, which combined the structured light with the marked points, and realized the rapid reconstruction of 3D point cloud of the human body and the automatic and rapid measurement of 3D attitude (distance and angle). The experimental results show that when the distance is more than 2 m and the measuring range is 1.5 m × 2 m, the measurement accuracy of human body posture can reach 0.03 mm, which meets the demand of high-precision three-dimensional data acquisition of automobile human body posture. Compared with traditional three-dimensional measurement method, the three-dimensional automatic measurement method used in this paper not only has a high degree of automation, but also has the advantages of high accuracy, fast speed and strong robustness.