Abstract: Relative spectral responsivity and the in-band spectral response non-consistency of polarization remote sensors are the basic parameters for laboratory calibration. In this paper, a spectrally-resolved calibration facility was assembled for the calibration of relative spectral responsivity by using novel supercontinuum laser and monochromator. Taking three polarization channels of 490 nm, 670 nm and 910 nm as examples, the relative spectral responsivity of the channel polarization remote sensors was obtained under the two states of the monochromator output light directly and the monochromatic light steered into an integrating sphere respectively, and the polarization state of the calibrated light source was measured under the two states. The experimental results show that there are obvious changes in degree of linear polarization (DOLP) and polarization orientation angle in the mode of direct output light of monochromator, and the non-consistency of in-band spectral response of the three polarization channels were 0.91%, 4.25% and 1.06%, respectively. When the monochromatic light steered into the integrating sphere, the DOLP of the light source decreases significantly, and the non-consistency of in-band spectral response were 0.15%, 0.47% and 0.57%, respectively, which indicates that depolarized light source can be effectively applied to the spectral responsivity calibration of polarization remote sensors. And the system-level calibration facility based on supercontinuum laser and monochromator has wide application prospects in the calibration of polarization remote sensors.