Abstract: An prototype of imaging spectrometer for the remote sensing of solar induced chlorphyll fluorescence of vegetation was researched to satisfy the scientific requirements of high SNR and high spectral resolution for the weak fluorescence observation. Based on the principle of relationship between the fluorescence and solar Fraunhofer lines, the optimization of the optical system with high performance was completed. It covered the visible-near infrared working waveband of 670-780 nm, which also covered the special wavelengths of the solar induced fluorescence. The numerical aperture was 0.25 which ensured the enough SNR of the system. The imaging spectrometer also owned spectral resolution better than 0.3 nm and excellent imaging quality. The performance tests of the prototype were studied. The research will supply an excellent engineering application in the further solar induced chlorophyll fluorescence high spectral imaging observation.