Abstract: MTF test is an important part of the camera manufacture process. General practice of MTF test needs to use the parallel light tube. Self-collimating MTF test method does not rely on the parallel light tube. Firstly, the principle and construction plan of the self-collimating MTF test system were introduced. Secondly, the curve measured by parallel light tube and self-collimating MTF test were compared. It is obtained that the self-collimating MTF test system has high sensitivity to the off-focus, this method can be used to determine the focal plane position. Thirdly, the phenomenon of the deflection of the self-collimating target fringe were analyzed, which will affect the MTF test result. The formula of the deflection angle of target fringe and the formula of the influence of fringe deflection on MTF test result were given. Design method of the self-collimating target of off-axis system were given. With this method, the deviation of MTF caused by the deflection of the target fringe can be reduced to a negligible level. Splicing relationship between the detector and the test target of self-collimating MTF test system were discussed, influence of detector's filter on focal plane position was analyzed, and design and splicing program of the detector and the target were proposed. Finally, the application of self-collimating MTF test on a camera were summarized. The result obtained by this study can be used to other camera in the future.