Abstract: The application of infrared imaging seeker in weapons systems is becoming wider. In its development process, a dynamic infrared scene simulator test equipment is commonly used in laboratories, but there is still no measurement standard of dynamic infrared scene simulators, thus the accuracy and reliability of test results can not be guaranteed. A calibration device for dynamic infrared scene simulators was presented in this paper, which realized calibration for spectral range, frame rate characters, radiation temperature, non-uniformity and distortion of dynamic infrared scene simulator, and achieved good results.