Abstract: Based on the real-time applications of EMCCD imaging system in low light environments, for the characters of output signal and the strict requirements of the EMCCD drive signals on phase, frequency and amplitude, a strategy after in-depth analysis about functions of each part of the analog front-end (AFE) was proposesd to improve the the EMCCD driver circuits, optimize the drive signals quality and reduce the noise level of EMCCD AFE. A real-time, low noise, stable EMCCD camera was designed, which can work in low light level situation. Testified by projects, when the ambient illumination is 1 10-3 lx, EMCCD camera has real-time and high-speed imaging capability, when the signal clock is 12.5 MHz, the image data rate is 25 frames per second, the output signal is stable and low noise, the captured images are clear, stable, basically meets the real-time applications requirements for the EMCCD imaging system in low light environments.