Abstract: By right of its non-diffracting and self-constructing property, Bessel beam has gained growing attentions from scholars since its naissance. Based on the generalized Lorenz-Mie theory (GLMT), the incident on-axis Bessel beam and scattered fields of the chiral sphere were expressed in terms of the spherical vector wave functions (SVWFs). The analytical solution to the scattering of chiral sphere illuminated by an on-axis zero-order Bessel beam was investigated by utilizing the SVWFs and continuous boundary conditions. The on-axis Bessel beam was degenerated to a plane wave incident on a chiral sphere, and the results were found to be in good agreement with the literature available. The effects of beam, particle parameters, and on-axis beam waist center shift z0 on the scattered intensity were numerically analyzed. The theory present here may play an important role in the fields of the applications of chiral material and chiral-coated target in microwave engineering and shielding targets.