Abstract: A novel method for multi-plane imaging in digital holography was proposed. A non-conjugated quadratic distorted phase factor was used to act on the experimental recorded digital hologram and the reconstructed images at multiple planes would be obtained simultaneously by only single Fresnel diffraction. It was firstly introduced the principles for multi-plane imaging using non-conjugated quadratic distorted phase factor and the effectiveness of the proposed method was verified by experiments. It needed single digital hologram of objects at different positions and multi-plane images could be rebuilt at arbitrary reconstruction distance by selecting the suitable parameters according to this paper's theory. The root mean square error(RMSE) and peak signal to noise ratio(PSNR) were used for evaluation standard of the image quality and reconstructed images at different positions were compared. Digital focusing at multi-planes can be achieved simultaneously without the symmetric optical design on the premise and the depth of focus for the Fresnel digital holographic reconstruction images can be extended.