Abstract: Stripe nonuniformity is a very typical fixed pattern noise in line infrared focal plane arrays (IR-FPA) and uncooled staring IR-FPA. In this paper a model was put forward based on the separation between stripe nonuniformity and real scene, and the correction could be achieved in a single frame. The estimated nonuniformity correction parameter was obtained by traversing the error function of adjacent column pixels in local template window. Through the succession of two adjacent columns' correlation, the stripe nonuniformity correction was achieved. By analyzing the experiment results of the simulated imagery and the real infrared image sequence, it is obvious that the proposed method can significantly enhance the performance of stripe nonuniformity correction.