Abstract: As to the change of 3D pose of dynamic target without tags, how to locate the target's pose on single camera mode rapidly and accurately was researched. On the premise of the knowledge of target's structure, the method of real-time pose location based on structural feature was proposed. On the basis of tracking steadily, the 2D feature of target in image which was caputured by optical camera was extracted. Corresponding the 3D features of the target with its 2D features in image, the mathmatics mode could be cancluted, and then the pose of dynamic target was located. The simulated experiment was developed with the imitation of airplane's flight. Applying this method on the imitation flight, the aveage time of processing was less than 0.02 s and the frequency of location was upper than 50 Hz. The experiment results show that this method is effective for pose real-time location of dynamic target.