Abstract: The in-flight radiometric calibration for thermal bands of HJ-1B was described using the test site, Qinghai-lake based on the calibration of the thermal-infrared field radiometer CE312. Because of the instability of the onboard calibration, the high temperature point was hard to obtain, the single-point and two-point calibration methods were used to calculate the calibration coefficients respectively. The results were validated by the data of June 29, 2010 at the Dali-lake test site. It shows that the calibration coefficients calculated by the data of Qinghai-lake have high reliability. And the comparison between the methods of single-point calibration and two-point calibration show that the accuracy of two-point calibration method is better than the single-point one.