Abstract: With the rapid economic development, the ambient air quality has been on the slide. Gas emission sources monitoring was an important research in the field of environment science. The high temperature reference spectral line model method and standard gas measurement method were put forward and realized, by which the synthetic calibration spectrum was corrected and the high temperature gas concentration retrieval accuracy was improved based on the nonlinear least square algorithm of numerical calculation. The concentrations of the standard gas CO were inversed under different temperatures by the experiment measured transmittance spectra. As a result, the accuracy has been improved greatly, comparing to concentration inversion results with no temperature correction calibration spectrum. The results in the paper were important for high temperature gas spectral features, perfecting quantitative analysis algorithm, accurately monitoring gas emissions concentration not at the room temperature, infrared guidance and so on.