Abstract: The column fixed pattern noise(CFPN) of the CMOS image sensors have severe influence on image quality. A column fixed pattern noise correction method for CMOS image sensors was proposed based on the analysis of emerge mechanism, noise characteristic and the behavior in the output image of the fixed pattern noise. It sampled the uniform and parallel light source which radiated from a integrating sphere more times use a CMOS image acquiring system and then estimated the column fixed pattern noise using these images. Then, the estimation result was used to correct the column fixed pattern noise in the CMOS image acquisition system. Experimental results show that with this method, the fixed pattern noise can be effectively reduced and the quality of the image can be increased.